Fig. 6
From: Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity
Astrocytic NFIA knockdown prevents TRPV4 upregulation and its calcium activity. A Scheme of primary astrocyte preparation for immunofluorescent staining and protein analysis. Representative confocal immunofluorescent images (Bi) of GFAP and TRPV4, and the immunofluorescent intensity analysis of GFAP (Bii) and TRPV4 (Biii) in the astrocytes transfected with si.NC or si.NFIA in the presence or absence of 4-AP (si.NC: n = 7, si.NFIA: n = 6, si.NC + 4-AP: n = 6, si.NFIA + 4-AP: n = 6, **P < 0.01, ****P < 0.001, One-way ANOVA). Ci Representative traces of Ca2+ indicator Fluo-4 fluorescence intensity changes in astrocytes responding to GSK1016790A, and the analysis (Cii) of the increased Fluo-4 fluorescence intensity peak, ΔF/F0, which was calculated as (F–F0)/F0. F0 is the mean value of fluorescent intensity before the addition of GSK1016790A (n = 30 cells for each group, *P < 0.05, ***P < 0.001, one-way ANOVA)