Fig. 7
From: Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity
Astrocytic NFIA transcriptionally regulates TRPV4 expression by direct binding to its promoter. A Representative immunoblot bands of NFIA and TRPV4 in primary cultured astrocytes transfected with NFIA overexpression plasmid (HA-NFIA) or vector plasmid (HA) and their density analysis (n = 3 for each group, *P < 0.05, Student’s t test). B ChIP assays showing binding of NFIA and TRPV4 promoter: left, the primer sets at the TPRV4 promoter; right, the qPCR analysis of the enrichment region of NFIA at the TRPV4 promoter sequence (n = 3, **P < 0.01, Student’s t test)