Fig. 8
From: Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity
Functional TRPV4 is required for NFIA-regulated astrocyte conversion. A Schema of overexpressing HA/HA-NFIA astrocyte preparation for exploring the role of TRPV4 functional activity. Representative confocal immunofluorescent images (Bi) of C3 and its immunofluorescent intensity analysis (Bii) in NFIA-overexpressing astrocytes with/without 4-AP exposure in the presence or absence of Hc, a TRPV4 antagonist (n = 6 for each group, ***P < 0.001, one-way ANOVA). (Ci) Representative immunoblot bands of NFIA, GFAP and C3, and the band density analysis of NFIA (Cii), GFAP (Ciii) and C3 (Civ) in NFIA-overexpressing astrocytes with/without 4-AP exposure in the presence or absence of Hc (n = 3 for each group, **P < 0.01, ***P < 0.001, one-way ANOVA). (Di) Representative immunoblot bands of TNF-α, IL-6 and IL-1β, and the band density analysis of IL-6 (Dii), TNF-α (Diii) and IL-1β (Div) in NFIA-overexpressing astrocytes with/without 4-AP exposure in the presence or absence of Hc (n = 3 for each group, **P < 0.01, ***P < 0.001, One-way ANOVA)