Fig. 9
From: Cell-specific NFIA upregulation promotes epileptogenesis by TRPV4-mediated astrocyte reactivity
Effects of hippocampal astrocyte-specific NFIA knockdown on 4-AP-induced seizure and neuronal damage in mice. A Scheme of experiments in 4-AP-induced seizure mouse model. 4-AP-induced behavioral observation including Bi seizure grade, Bii seizure latency and Biii seizure duration in mice with hippocampal delivery of rAAV- GfaABC1D-sh.NC (n = 6) or rAAV-GfaABC1D-sh.NFIA (n = 5), *P < 0.05, **P < 0.01, Student’s t test. Representative EEG recordings (C) in mice infected with rAAV-GfaABC1D-sh.NC (n = 6 for both ip. saline and ip. 4-AP group) or rAAV-GfaABC1D-sh.NFIA (n = 5 for both ip. saline and ip. 4-AP group). HE (D) and Nissl (E) staining in the hippocampal tissues from the mice infected with rAAV-GfaABC1D-sh.NC (n = 6 for both ip. saline and ip. 4-AP group) or rAAV-GfaABC1D-sh.NFIA (n = 5 for both ip. saline and ip. 4-AP group), showing 4-AP-induced neuronal damage in DG and hilus from the mice infected with rAAV-GfaABC1D-sh.NFIA is significantly alleviated compared with that in rAAV-GfaABC1D-sh.NC-infected group, **P < 0.01, ****P < 0.0001 one-way ANOVA