Table 1 Antibodies applied in immunohistochemical or immunofluorescent assay

Primary antibody

 Anti-NFIA

Abcam (ab228897)

1:1000 (WB)

1:200 (IF)

 Anti-TRPV4

Abcam(ab2040264)

1:500 (WB)

1:200 (IF)

 Anti-GFAP

CST(#3670)

1:1000 (WB)

1:200 (IF)

1:200 (IHC)

 Anti-C3

Proteintech (21,337–1-AP)

1:1000 (WB)

1:200 (IF)

1:1000 (WB)

 Anti-S100A10

Proteintech (11,250–1-AP)

1:200 (IF)

 Anti-IL-6

Bioss(bs-0782R)

1:1000 (WB)

 Anti-IL-1β

CST(#12242S)

1:500 (WB)

 Anti-TNFα

ABclonal(A0277)

1:1000 (WB)

 Anti-HA

CST(C29F4)

1:500 (WB)

Secondary antibody

 Goat anti-mouse IgG

Biosharp(BL001A)

1:10,000 (WB)

 Goat anti-rabbit IgG

Biosharp(BL003A)

1:10,000 (WB)

 HRP-conjugated anti-mouse antibody

Abbkine (A21010)

1:500 (IHC)