Fig. 1

Amyloid-beta plaque burden in EC and DCN of App KI mice. A Immunohistochemistry of brain sections from 6-month-old App^NL-G-F mice using 6E10 antibody to detect Aβ plaques (red), IBA1 to detect microglia (green), and DAPI to stain nuclei (blue). Images were taken at 20X magnification. Left: regions of interest (ROIs) are indicated on whole brain sections: entorhinal cortex (EC, a), cerebellar cortex (CBX, b) and deep cerebellar nuclei (DCN, c). Scale bar at 1000 µm. Right: ROIs with an example of an Aβ plaque in each region indicated and enlarged in the top-right corner. Scale bar at 100 µm. B Density of 6E10⁺ positive plaques per mm² in the EC, CBX and DCN using size filters of > 10 μm² and > 100 μm². Quantification was performed on N = 3 subjects, n = 3 sections/subject. Graphs indicate mean ± SEM. C Quantification of the average size of 6E10⁺ plaques per mm² using size filters of > 10 μm² and > 100 μm². Graphs indicate mean ± SEM. Two-way ANOVAs were performed on within-subject means to test for main effects of region and genotype and interaction effects, followed by Tukey HSD post hoc tests indicated by currency sign (¤). Asterisks (*) indicate within-timepoint differences between regions. Daggers (†) above bar indicate between-timepoint differences within regions. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001