Fig. 4

Multiple cell types in the DCN contribute to a cytokine-enriched microenvironment. A Heatmap showing pseudo-bulk average expression of ‘cytokine activity’ genes (GO:0005125) for each major cell type in each condition. B UMAPs of expression of representative DCN-enriched cell-type-specific cytokines. Colour scale indicates log-normalised expression. C Enrichment of ligand–receptor interactions associated with GO biological processes ‘immune system process’ (GO:0002376) and ‘inflammatory response’ (GO:0006954) in WT EC compared to DCN. Nodes represent glial cell types and edges represent ligand–receptor pairs with greater combined expression in each region. Edge thickness indicates number of pairs and edge colour indicates ligand cell type. D Top 50 ligand–receptor interactions associated with immune and inflammatory pathways between AST1 ligands and microglia receptors. Node colour and border indicate differential expression in WT DCN vs EC. E As in D for AST1 receptors and microglia ligands