Fig. 3

Aβ oligomers internalization and clearance is independent on microglial IDE genotype. A FAM-Aβ oligomers internalization measured by flow cytometry after different exposure times (0.5–3 h). Four independent experiments (2–4 samples/time point) were analyzed. Normalized average fluorescence change, and % of Aβ positive cells are represented. Histograms of the experiment with the highest difference between genotypes show a mild, transitory acceleration of endocytosis. The control histogram without FAM-Aβ exposure defines the fluorescence threshold to classify an event as FAM-Aβ positive microglia. Numbers depict the percentage of FAM-Aβ( +) cells. Arrows point out the greatest differences. B Time course of Aβ oligomers clearance measured by flow cytometry in cells exposed to FAM-Aβ oligomers for 3 h (internalization period), followed by several degradation times (0–24 h). Three independent experiments with at least 10,000 cells per sample and 2–4 samples/time point were analyzed. Normalized average fluorescence change, and % of Aβ positive cells are represented. Histograms of the experiment with the highest difference between genotypes shows a transitory deceleration of clearance. No statistical differences are found at any time point in both assays. Statistical differences were assessed by 2-way ANOVA considering the factors genotype and time, followed by post-hoc Holm–Sidak comparisons. Endocytosis mean FL [genotype p = 0.078; time p = 0.0003]; endocytosis %Aß( +) cells [genotype p = 0.534; time p = 0.0252]; clearance mean FL [genotype p = 0.5636; time p = 0.0333]; clearance %Aß(+) cells [genotype p = 0.6752; time p = 0.0155]