Table 2 Primary antibodies and blocking buffers for immunohistochemistry
From: Mertk-expressing microglia influence oligodendrogenesis and myelin modelling in the CNS
Target | Host species | Dilution | Manufacturer, catalogue # | Blocking buffer |
|---|---|---|---|---|
Mertk | Rat | 1:50 | eBioscienceâ„¢, DS5MMER | 0.1% X-100 Triton/10% normal serum in MT-PBS |
Iba1 | Rabbit | 1:1000 | Wako, 019-19741 | 0.3% X-100 Triton/10% normal serum in MT-PBS |
PDGFRα | Goat | 1:50 | R&D Systems, AF1062 | |
CC1 | Mouse | 1:100 | Merck Millipore, OP80 | |
dMBP | Rabbit | 1:500 | Sigma-Aldrich, AB5864 |