Fig. 4

Cerebrospinal (CSF) white blood cell (WBC) counts at 24 and 42 h post-infection in TAM-treated PDGFRB::creER2-iDTA mice (PDGFRβ-iDTA) that are largely missing pericytes compared to control groups (A). Prime™ PCR array analysis of cDNA obtained from brains of infected transgenic mice revealed higher expression levels of CXCL1, CXCL2, and CCL2 (and also of TLR2 and ARG1), but lower PDGFRβ expression following pericyte depletion (B). The array results were confirmed by supplemental RT-PCR analyses (C), which showed increased brain expression of CXCL1, CXCL2, and CCL2 in TAM-treated PDGFRB::creER2-iDTA mice. Data are given as individual values as well as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, using ANOVA with Tukey’s multiple comparisons test