Fig. 5

A The percentage of Ly6C-high monocytes on day 1 post-surgery was assessed by flow cytometry (n = 6/group). B Schematic diagram of the establishment of chimeric mice. C, D The percentage of GFP-positive Ly6C-high monocytes on day 1 post-surgery was assessed by flow cytometry (n = 6). E, F The percentage of GFP-positive cells in the hind limb and calvaria of the wild-type (WT) and chimeric mice on day 1 post-surgery was assessed by flow cytometry (n = 2). G–L The percentage of Ki-67-positive and associated mean fluorescence intensity (MFI) of MDPs, cMoPs, and Ly6C-high monocytes on day 1 post-surgery was assessed by flow cytometry (n = 5/group). M, N Schematic diagram of intracalvarial bone marrow injection and GFP-positive ratios in neutrophils and Ly6C-high monocytes on day 1 post-surgery was assessed by flow cytometry. GFP-positive ratio = brain GFP-positive ratio/skull GFP-positive ratio, n = 6. ns, P ≥ 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001