Fig. 1

Elevated Lrg1 expression in multiple cell types in brain tissue during cerebral ischemia‒reperfusion injury. A Western blotting was used to determine total Lrg1 expression in brain tissues during different durations in MCAO/R mice. Mice were exposed to 0.5 and 1 h of ischemia and 1, 6, 12, and 24 h of reperfusion. Data are the mean ± SD, n = 8. ^##p < 0.01 vs. sham group. B The UMAP plot presents the single-cell atlas of brain tissue after cerebral ischemia‒reperfusion injury. C The heatmap illustrates the expression of recognized marker genes in different cell types. D The violin plot displays Lrg1 expression in different cell types. E Immunofluorescence staining verified Lrg1 expression (red) in endothelial cells, neurons, and microglial cells in mouse brain tissues after MCAO/R. Scale bar = 50 μm. F The dot plot presents the differential expression analysis results of Lrg1 in various cell types between the MCAO/R and sham groups. An average log fold change (avg_logFC) value > 0 indicates that Lrg1 is expressed at higher levels in the MCAO/R group than in the sham group