Fig. 4

Lrg1 knockout reduces BBB dysfunction after cerebral ischemia‒reperfusion injury in mice. A Venn diagram illustrating the overlapping differentially expressed genes in BBB constituent cells (upregulated in brain tissue of Lrg1^−/− mice after cerebral ischemia‒reperfusion injury compared to WT mice). B Pathway enrichment analysis results of the 99 overlapping differentially expressed genes of BBB constituent cells. C Violin plots displaying the expression of intercellular adhesion molecules in BBB constituent cells of different groups and colored according to the standardized expression median. D–G Representative immunofluorescence images showing the protein expression of Claudin 11, Annexin A2, Integrin β5, and Protocadherin 9 in the brain tissues of mice from different groups. Scale bar = 20 μm. H Microvascular albumin leakage test showing the change in albumin leakage in the venules of the brain tissues from different mouse groups. I Error bar graph showing the results of the microvascular albumin leakage test in different groups. Data are expressed as the mean ± SD, n = 8. Scale bar = 400 ×