Fig. 5

Lrg1 knockout alters the functional state of microglial cells and macrophages after cerebral ischemia‒reperfusion injury in mice. A Violin plot showing the expression of functional molecules in microglial cells of different groups. The coloring is based on the standardized median expression of each group. B Violin plot exhibiting the expression of functional molecules in macrophages of different groups. The coloring is based on the standardized median expression of each group. C Representative images showing interleukin 6 protein expression in the brain tissues of mice from different groups. Scale bar = 20 μm. D Representative images showing tumor necrosis factor α protein expression in the brain tissues of mice from different groups. Scale bar = 20 μm. E Box plot showing the functional state score of microglial cells from different groups. F Pathway enrichment analysis of upregulated differentially expressed genes in microglial cells in brain tissues of Lrg1^−/− mice after MCAO/R compared with WT mice after MCAO/R