Fig. 6
Lrg1 knockout affects microglial cell maturation after cerebral ischemia‒reperfusion injury in mice. A The UMAP plot illustrates the clustering results of microglial cells in brain tissue after MCAO/R. B The heatmap depicts the expression of the top 50 differentially expressed genes in distinct microglia subpopulations. C The box plot demonstrates the M1 score in microglial cells across different clusters. D The box plot exhibits the M2 score in microglial cells across different clusters. E The box plot displays the phagocytosis scores in microglia across different clusters. F The dot plot demonstrates the distribution of different clusters in the differentiation trajectory plot of microglial cells in Sham + WT group constructed using monocle2. G The dot plot illustrates the differentiation trajectories of microglial subpopulations in Sham + WT group drawn by monocle2. The direction of arrows indicates the direction of differentiation and development. H The dot plot illustrates the differentiation trajectories of microglial subpopulations in Sham + WT group drawn by CytoTRACE. The direction of arrows indicates the direction of differentiation and development. I Heatmap visualizations depicted the enrichment profiles of distinct cell clusters (rows) across diverse groups (columns). The coloration within the cells corresponds to the Ro/e values, denoting the extent of cell cluster enrichment as delineated below: Insufficiency: Ro/e ≤ 1; Limited Enrichment: 1 < Ro/e ≤ 1.5; Intermediate Enrichment: 1.5 < Ro/e ≤ 3; Substantial Enrichment: Ro/e > 3. J The heatmap illustrates the transcription factors of Path 1 that change with the trajectory in Sham + WT group. Different TF clusters signify TFs with varying expression patterns along the trajectory. K The heatmap demonstrates the transcription factors of Path 2 that change with the trajectory in Sham + WT group. Different TF clusters represent TFs with distinct expression patterns along the trajectory