Fig. 1

Treatment with GTA increased lipogenesis in the peri-infarct area on day 3 after cerebral ischemia. A Free lauric acid level in the peri-infarct area. B Free palmitic acid level in the peri-infarct area. C Free stearic acid level in the peri-infarct area. D The protein level of FASN in the peri-infarct area. E The expression of FASN in astrocytes in the peri-infarct area. F Nile red staining of tissue lipids in the peri-infarct area. G Lipidspot staining of tissue lipids in the peri-infarct area. H Relative Nile red fluorescence intensity. I Lipid droplets concentration based on Nile red (F). J Relative Lipidspot fluorescence intensity. K Lipid droplets concentration based on Lipidspot (K). n = 6 for A–C, n = 4 for D, n = 5 for H–K. Compared with Sham, *P < 0.05, **P < 0.01. Compared with MCAO, ^#P < 0.05, ^##P < 0.01 (tested by one-way ANOVA with post hoc Tukey method). Scale bar = 50 μm in E, scale bar = 10 μm in F and G