Fig. 3

OGT, OGA and O-GlcNAcylation changes following SD or RSD. The zebrafish brains were sacrificed for analysis after the induction of SD, SD(R), or RSD. A–C Representative confocal images (× 40) of DAPI (blue), O-GlcNAc (A, green), OGA (B, green), OGT (C, green), and merged immunofluorescence staining are shown for the Dl region of the zebrafish telencephalon. Enlarged images are presented within white boxes. The graphs represent the quantitative results for each antibody, normalized to the DAPI levels (n = 3 ~ 6/group). D mRNA expression levels of pooled brain samples (n = 3/group) were measured using quantitative real-time PCR with specific primers. Data represent the mean ± SEM of target gene expression, normalized to β-actin levels using the delta–delta Ct method. The experiments were independently replicated three times. E Representative Western blot images of O-GlcNAc, OGT, OGA, and β-actin from zebrafish whole brains (n = 9/group). The graphs represent the quantitative results for each antibody, normalized to the β-actin levels. For statistical analysis, the Mann–Whitney test was performed for the two group comparisons, while the Kruskal–Wallis test with the original Benjamini and Hochberg false FDR was used for comparisons involving more than three groups (*p < 0.05, **p < 0.01 versus Con)