Fig. 6

Clec4e silencing suppresses the proinflammatory effect of properdin on microglia. a Experimental design for Clec4e silencing in primary microglia. b The transfection efficiency of lentivirus in microglia was determined by qPCR. n = 3 per group, **P < 0.01 versus the Lv-NC group, one-way ANOVA with Bonferroni post hoc test. c Western blotting analysis of Mincle expression after Clec4e silencing. n = 3 per group, *P < 0.05 versus the Lv-NC + properdin group, one-way ANOVA with Bonferroni post hoc test. d Protein expression of p-Syk, p-p65 and C/EBPβ in microglia after silencing Clec4e. e Quantification of the p-Syk/Syk ratio, p-p65/p65 ratio and C/EBPβ expression was normalized to GAPDH expression. n = 3 per group, *P < 0.05 versus the Lv-NC + properdin group, **P < 0.01 versus the Lv-NC + properdin group, one-way ANOVA with Bonferroni post hoc test. f mRNA levels of Il1b, Tnf, Nos2 and Il10 in microglia after Clec4e knockdown. n = 3 per group, *P < 0.05 versus the control group, **P < 0.01 versus the control group, ***P < 0.001 versus the control group, ^#P < 0.05 versus the Lv-NC + properdin group, ^##P < 0.01 versus the Lv-NC + properdin group, one-way ANOVA with Bonferroni post hoc test. g Images of calcein-AM/PI staining showing the level of neuronal death. The data were quantified as the percentage of calcein-AM-positive cells. n = 5 per group, ***P < 0.001 versus the control group, ^##P < 0.01 versus the Lv-NC + CM group, one-way ANOVA with Bonferroni post hoc test