Fig. 1

NfL expression in various anatomical locations and NfL release in MOG-EAE. A Cartoon depicting the investigated murine brain regions in adult mouse brains (red boxes). B Comparison of the respective CNS region sizes as percent of total brain volume. C Representative western blot for NfL expression in the respective CNS regions and spleen; β-Tubulin as control. D Quantification of the results shown in C. One-way ANOVA with Dunnett’s multiple hypothesis test. n ≥ 3. E Experimental workflow: mice were randomly assigned to an early (stable neuro-score 3 days after peak) and a late cohort (stable neuro-score 20 days after peak). F Graph depicting the mean and SEM EAE scores. n = 18. Specific time-points are indicated in red. G Linear regression and Pearson correlation of serum NfL (sNfL) and CSF NfL levels of early and late EAE combined. n = 18. H Pearson correlation of serum NfL (sNfL) and CSF NfL levels with the EAE score. n = 14. Images were created with BioRender and servier medical art. I Bar graph showing sNfL levels measured with SiMoA in pg/ml of early and late chronic EAE (n = 8 per condition, two-tailed Student’s t-test). *p < 0.05, **p < 0.01, ***p < 0.001