Fig. 6

Co-cultures of neuroaxons and CNS-myeloid cells. A Cartoon depicting the experimental workflow of neuroaxonal-CMC co-cultures. Act. microgl. sup.: activated microglia supernatant. Image was created with BioRender. B Representative images of neuroaxonal-CMC co-cultures. Neurons were stained with Tuj1 (green), CMCs were stained with Iba1; nuclear counter-stain with DAPI. Scale bar: 100 µm. Arrows indicate microglia in close contact with axons. C Quantification of NfL release in neuroaxonal-CMC co-culture supernatants, relative to control (neuron culture medium) (n = 6–10, per condition). One-way ANOVA with post hoc Dunnet’s test. Mean and SEM