Fig. 10

Myelin survival in the nimodipine-treated spinal cord. A Black Gold II staining in representative coronal sections of lumbar spinal cords. Tissue was collected from untreated subjects and EAE mice treated with vehicle or nimodipine during the acute or peak phase of the disease. Scale bar, 90 μm. B Demyelinated lesions were measured as a percentage of the dorsal, ventral, and total WM area. C Representative western blots for MBP, CNP, PLP, and MOG in the lumbar spinal cord of untreated subjects and EAE mice treated with vehicle or nimodipine during the acute or peak phase of the disease. α-Tubulin and β-actin were used as internal standards. One-way ANOVA followed by the Bonferroni’s test was used for comparisons between experimental groups. Analysis consisted of four spinal cords per condition and each dot denotes the mean of one subject. Values are presented as mean ± SEM ^^p < 0.01, ^^^p < 0.001 versus untreated; **p < 0.01, ***p < 0.001 versus vehicle; ##p < 0.01 versus onset