Fig. 4

Myelin survival in the Cav1.2^KO spinal cord. A Black Gold II staining in representative coronal sections of lumbar spinal cords. Tissue was collected from untreated, control and Cav1.2^KO mice at D39. Scale bar, 90 μm. B Demyelinated lesions were measured as a percentage of the dorsal, ventral, and total WM area. C Representative western blots for MBP, CNP, PLP, and MOG in the lumbar spinal cord of untreated, control, and Cav1.2^KO mice. α-Tubulin was used as internal standard. One-way ANOVA followed by the Bonferroni’s test was used for comparisons between experimental groups. Analysis consisted of at least four spinal cords per condition and each dot denotes the mean of one subject. Values are presented as mean ± SEM ^^p < 0.01, ^^^p < 0.001 versus untreated; *p < 0.05, **p < 0.01, ***p < 0.001 versus control