Fig. 5

Reduced astrocyte and microglia reactivity and lymphocyte infiltration in the Cav1.2^KO spinal cord. A Representative coronal sections of lumbar spinal cords immunostained for GFAP, Iba1, and CD45. Tissue was collected from untreated, control, and Cav1.2^KO mice at D39. Scale bar, 90 μm. B Density of GFAP-positive cells and integrated fluorescence intensity of Iba1 and CD45 were quantified in the spinal gray matter and ventral white matter (WM). C Representative western blots for GFAP and Iba1 in the lumbar spinal cord of untreated, control, and Cav1.2^KO mice. α-Tubulin and β-actin were used as internal standards. D qPCR analysis of TGFβ, IFNγ, IL1β, IL6, and IL10 in spinal cord samples from untreated, control, and Cav1.2^KO mice at D39. GAPDH and TBP were used as internal standards and values are expressed as fold change of untreated values ± SEM. One-way ANOVA followed by the Bonferroni’s test was used for comparisons between experimental groups. Analysis consisted of at least four spinal cords per condition and each dot denotes the mean of one subject. Values are presented as mean ± SEM ^p < 0.05, ^^p < 0.01, ^^^p < 0.001 versus untreated; **p < 0.01, ***p < 0.001 versus control