Fig. 9

Reduced demyelination in the nimodipine-treated spinal cord. A Representative coronal sections of lumbar spinal cords immunostained for PLP, MBP, and Olig2. Tissue was collected from untreated subjects and EAE mice treated with vehicle or nimodipine during the acute or peak phase of the disease. Scale bar, 90 μm. B Demyelinated lesions were measured as a percentage of the dorsal and ventral WM area using PLP and MBP. C Olig2- and CC1-positive cell density were measured in the entire spinal section. One-way ANOVA followed by the Bonferroni’s test was used for comparisons between experimental groups. Analysis consisted of at least four spinal cords per condition and each dot denotes the mean of one subject. Values are presented as mean ± SEM ^^p < 0.01, ^^^p < 0.001 versus untreated; **p < 0.01, ***p < 0.001 versus vehicle