Fig. 3

ND1–GFP improved the cellular microenvironment and boosted neurogenesis following SAH. A Schematic illustration of the experimental protocol. B Representative immunofluorescent image showed the whole brain immunostaining for Nestin, and the magnified image revealed that the intensity of Nestin was significantly higher in the ND1–GFP side (L) than that in the control GFP side (R) at 14 dpi. Scale bar: 500 μm for whole brain section 50 μm for the enlarged images. L, left. R, right. C Representative immunofluorescent image showed the whole brain immunostaining for Ki-67 and Nestin, and the enlarged images showed that ND1–GFP-boosted Nestin expression and promoted Ki-67 labeled cell proliferation in the subgranular zone known for neurogenesis. Scale bar: 500 μm for whole brain section 50 μm for the enlarged images. L, left. R, right. D Representative immunofluorescent image showed the expression of Nestin in mice with or without ND1–GFP treatment. Scale bar, 25 μm. E Representative immunofluorescent image revealed the number of SOX2⁺Ki-67⁺ cells in the hippocampus in mice with or without ND1–GFP treatment. Scale bar, 25 μm. F, G Representative western blot image and quantification analysis of PTN in the hippocampus at 14 dpi. N = 6. ^**p < 0.01 indicated comparison with SAH + GFP group. H Representative immunofluorescent image showed the expression of DCX in the hippocampus at 14dpi after NeuroD1 treatment. N = 6. Scale bar: 100 μm