Fig. 1

IL-17A deficiency exacerbated disease severity in a laser-induced CNV mouse model. A Representative fundus fluorescein angiography (FFA) images from WT and IL-17A^−/− mice at 7, 14, 21, and 28 days after laser photocoagulation. CNV lesions were analyzed by fluorescence angiography. B Quantification of the CNV area after laser photocoagulation at 7, 14, 21, and 28 days. Statistical differences were determined by unpaired t test. Data are presented as the mean ± SEM (*P < 0.05, ** < 0.01, *** < 0.001). N = 18 mice per group. C H&E staining of the retinal structure in healthy mice and WT and IL-17A^−/− mice with laser-induced CNV at 28 days after laser injury. Magnification: 200X. Scale bar = 100 μm. D–J Representative flow cytometry data for the CD4⁺ T cell, γδ T cell, CD3⁺CD4⁺RORγt⁺ T cell, Treg cell, macrophage, neutrophil, and MDSC populations in the eyes of WT and IL-17A^−/− mice 28 days after laser injury. N = 5 mice per group. Statistical differences were determined by two-way ANOVA and Sidak’s multiple comparisons test. Data are presented as the mean ± SEM (*P < 0.05, **P < 0.01)