Fig. 2

Cellular source of IL-17A in WT mice with the laser-induced CNV. A Immunofluorescence staining of ocular tissue with an anti-IL-17A antibody in vivo. Rabbit IgG isotype was used as the negative control. Magnification: 200X. Scale bar = 50 μm. B Serum IL-17A levels were measured by using ELISA at 7, 14, 21 and 28 days after laser photocoagulation. N = 5 mice per group. C–E Flow cytometry analysis of IL-17A-expressing cells among ocular cells and further gating of immune cells (CD45⁺) and nonimmune cells (CD45⁻). Statistical differences were determined by one-way ANOVA and Tukey’s multiple comparisons test. Data are presented as the mean ± SEM (*P < 0.05, **P < 0.01). N = 8 mice per group. F–K Representative flow cytometry data for the CD3⁺IL-17A⁺ T cell, CD3⁺CD4⁺IL-17A⁺ T cell, CD3⁺CD8⁺IL-17A⁺ T cell, γδ T cell, Th17 cell, and Treg cell populations in the eyes of WT mice at 7, 14, 21, and 28 days after laser injury in comparison with healthy control mice. N = 8 mice per group. Statistical differences were determined by one-way ANOVA and Tukey’s multiple comparisons test. Data are presented as the mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001). The data shown are representative of three independent experiments with similar results. L The dynamic changes in the proportion of IL-17A-expressing immune cells and the percentages of nonimmune cells, Treg cells, Th17 cells, and γδ T cells were analyzed at 7, 14, 21, and 28 days after laser injury. N = 8 mice per group