Fig. 4

IL-17A inhibited H₂O₂-induced apoptosis through HO-1 in ARPE-19 cells. A, B ARPE-19 cells were treated with IL-17A (10-100 ng/ml), H₂O₂ (300 μM), and IL-17A (100 ng/ml) plus H₂O₂ (300 μM) for 24 h. Cell viability was analyzed using the MTT assay. Statistical differences were determined by one-way ANOVA and Tukey’s multiple comparisons test. Data are presented as the mean ± SEM (*P < 0.05, **P < 0.01). C ARPE-19 cells were treated with or without IL-17A (100 ng/ml) for 24 h and then treated with H₂O₂ (300 μM) for 24 h, and cell apoptosis was analyzed using FACS. D Quantification of Annexin V⁺/ PI⁺ ratios in ARPE-19 cells after H₂O₂ and IL-17A treatment. Statistical differences were determined by one-way ANOVA and Tukey’s multiple comparisons test. Data are presented as the mean ± SEM (**P < 0.01, ****P < 0.0001). The data shown are representative of three independent experiments with similar results. E, F ARPE-19 cells were cotreated with H₂O₂ and IL-17A for 8 h, and the mRNA levels of HO-1 and NQO-1 were analyzed by RT‒qPCR with specific primers. N = 3. Statistical differences were determined by one-way ANOVA and Tukey’s multiple comparisons test. Data are presented as the mean ± SEM (*P < 0.05, **P < 0.01, ****P < 0.0001). The data shown are representative of three independent experiments with similar results. G, H ARPE-19 cells were cotreated with H₂O₂ and IL-17A for 24 h. The cell lysates were collected and analyzed using immunoblotting with specific antibodies against Nrf2, HO-1, and NQO-1. Tubulin was used as an internal control. Statistical differences were determined by one-way ANOVA and Tukey’s multiple comparisons test. Data are presented as the mean ± SEM of three independent experiments. (*P < 0.05, **P < 0.01, ****P < 0.0001)