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Table 1 Absolute ultrastructural analysis of MG/MDM far from and near the SCI injury site in the lower thoracic dorsal column of 8-week-old SCI female mice

From: Microglia/macrophages are ultrastructurally altered by their proximity to spinal cord injury in adult female mice

 

Far SCI

Mean ± SEM (Min–Max)

Near SCI

Mean ± SEM (Min–Max)

Primary lysosomes (n)

0.000 ± 0.000 (0.000–0.000)

0.02381 ± 0.02381 (0.000–1.000)

Secondary lysosomes (n)

0.02500 ± 0.02500 (0.000–1.000)

0.07143 ± 0.04022 (0.000–1.000)

Tertiary lysosomes (n)

0.05405 ± 0.05405 (0.000–2.000)

0.07143 ± 0.05272 (0.000–2.000)

All lysosomes (n)

0.08108 ± 0.05976 (0.000–2.000)

0.1667 ± 0.06745 (0.000–2.000)

Lipid bodies (n)

0.05405 ± 0.03769 (0.000–1.000)

0.1190 ± 0.05058 (0.000–1.000)

Non-altered mitochondria (n)*

7.459 ± 1.458 (0.000–46.00)

11.76 ± 1.734 (0.000–60.00)

Altered mitochondria (n)*

0.7750 ± 0.2594 (0.000–9.000)

1.326 ± 0.2711 (0.000–9.000)

Altered mitochondria (%)

6.768 ± 1.805 (0.000–37.50)

13.76 ± 3.508 (0.000–100.0)

Elongated mitochondria (n)

0.4250 ± 0.1285 (0.000–4.000)

0.4783 ± 0.1193 (0.000–4.000)

Elongated mitochondria (%)

6.859 ± 3.747 (0.000–100.0)

1.970 ± 0.5291 (0.000–12.50)

All mitochondria (n)*

8.622 ± 1.777 (0.000–59.00)

13.50 ± 1.949 (0.000–65.00)

Non-dilated endoplasmic reticulum (n)***

13.41 ± 1.887 (2.000–65.00)

28.79 ± 2.8383 (2.000–82.00)

Dilated endoplasmic reticulum (n)

0.2432 ± 0.08133 (0.000–2.000)

0.8810 ± 0.2752 (0.000–9.000)

All endoplasmic reticulum (n)***

13.65 ± 1.890 (2.000–65.00)

29.67 ± 3.531 (2.000–85.00)

% Dilated endoplasmic reticulum (n)

2.187 ± 0.7448 (0.000–16.67)

2.645 ± 0.7405 (0.000–22.22)

Partially digested phagosomes (n)*

0.5676 ± 0.2210 (0.000–7.000)

1.286 ± 0.2668 (0.000–7.000)

Fully digested phagosomes (n)

0.6757 ± 0.1820 (0.000–4.000)

3.190 ± 1.050 (0.000–38.00)

Myelin phagosomes (n)***

1.400 ± 0.2722 (0.000–5.000)

5.857 ± 1.024 (0.000–22.00)

All phagosomes (n)***

2.649 ± 0.5100 (0.000–12.00)

10.33 ± 1.829 (0.000–59.00)

Association with non-degraded myelinated axons (n)

2.108 ± 0.3773 (0.000–9.000)

2.310 ± 0.4641 (0.000–15.00)

Association with degraded myelinated axons (n)

0.7027 ± 0.1683 (0.000–3.000)

1.810 ± 0.4287 (0.000–12.00)

Association with all myelinated axons (n)

2.811 ± 0.4945 (0.000–12.00)

4.119 ± 0.6468 (0.000–16.00)

Association with degraded myelin (%)

22.34 ± 5.694 (0.000–100.0)

38.58 ± 6.355 (0.000–100.0)

Axon terminals (n)****

1.189 ± 0.2081 (0.000–4.000)

0.09524 ± 0.04584 (0.000–1.000)

Dendritic spines (n) **

0.1892 ± 0.07591 (0.000–2.000)

0.000 ± 0.000 (0.000–0.000)

All synaptic contacts (n)****

1.270 ± 0.2589 (0.000–6.000)

0.07143 ± 0.04022 (0.000–1.000)

Glycogen granules (%)

0.000 ± 0.000 (0.000–0.000)

9.524 ± 4.584 (0.000–100.0)

Autophagosomes (n)

0.1081 ± 0.06465 (0.000–2.000)

0.3571 ± 0.1732 (0.000–7.000)

Cell area (µm2)*

28.36 ± 2.737 (10.56–107.6)

43.44 ± 4.287 (12.77–123.2)

Cytoplasmic area (µm2)***

11.94 ± 2.210 (2.242–79.54)

26.95 ± 3.352 (3.143–94.07)

Nucleus area (µm2)

16.42 ± 0.9003 (4.500–28.08)

16.49 ± 1.568 (5.122–47.20)

Cell perimeter (µm)

31.71 ± 3.048 (13.75–110.4)

38.96 ± 3.206 (15.97–109.4)

Circularity (a.u.)

0.4457 ± 0.03066 (0.08600–0.7900)

0.4129 ± 0.02687 (0.1280–0.7740)

Aspect ratio (a.u.)

2.217 ± 0.1408 (1.034–4.942)

2.003 ± 0.1251 (1.085–4.948)

Solidity (a.u.)

0.7935 ± 0.02276 (0.3790–0.9680)

0.7852 ± 0.02001 (0.4860–0.9540)

  1. n number, a.u. arbitrary unit
  2. The p values of statistically significant tests are highlighted with an asterisk symbol. Data reported is expressed as means ± SEM as well as the minimum and maximum values obtained. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using a non-parametric Mann–Whitney test for data that did not pass normality (using Shapiro–Wilk test) and parametric t test with Welsh’s correction. Statistical tests were performed on n = 10–14 MG/MDM per animal with N = 3 mice/group, for a total of 79 cell bodies analyzed
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Journal of Neuroinflammation

ISSN: 1742-2094