Fig. 5

Quantification of α-SMA + tissue within scala tympani of the base of the cochlea following cochlear implantation. Following 7-day feeding on chow with 1200 ppm of PLX-5622 (PLX) or control chow (No PLX), cochlear implantation was performed in CX3CR1^+/GFP Thy1^+/YFP mice. Respective diets were resumed following recovery from surgery. Electrical stimulation was continued until 28 days post-CI. Mice were euthanized at the desired endpoints (10, 28, or 56 days). Following euthanasia, 30 µm mid-modiolar sections were labeled with antiα-SMA antibodies. A–L Representative images are shown. Asterisks within the images indicate the tract of the CI, M analyses the data. The volume of the scala tympani and α-SMA + tissue volumes were measured using IMARIS image analysis software. Fibrosis was measured by dividing the volume of α-SMA + tissue by the volume of scala tympani, expressed in % volume. Number of cochlea used in this study are as follows: at day 10 post-CI, implanted ‘no PLX’ (n = 4), implanted PLX, (n = 3), contralateral ‘no PLX’ (n = 3), contralateral PLX(n = 5); at day 28 post-CI, Implanted ‘no PLX’ (n = 6), implanted PLX, (n = 5), contralateral ‘no PLX’ (n = 3), contralateral PLX(n = 4); at day 56 post-CI, implanted ‘no PLX’ (n = 3), implanted PLX, (n = 6), contralateral ‘no PLX’ (n = 3), contralateral PLX (n = 4). Error bars indicate SEM