Fig. 4
DAnergic neurodegeneration in Nurr1cko mice. A Representative immunoblots and quantitative analysis B of TH level in the midbrain of 12-month-old Nurr1cKO and Nurr1cWT mice (n = 3 mice per each group) after LPS or PBS treatment. C HPLC analysis of DA level in the midbrain of mice after LPS or PBS injection (n = 3 mice per each group). D Double-label immunostaining of α-synuclein (green) and TH (red) in the SNc of the 12-month-old mice after PBS or LPS treatment. Scale bar = 20 μm. E The proportion of α-synuclein positive and TH-positive neurons (α-synuclein+-TH+) in the SNc was quantified (n = 3 mice per group). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Two-way ANOVA Tukey test