Fig. 5

NLRP3 inflammasome is activated in the microglia of Nurr1^cKO mice. A Representative images with TH (red), Iba-1 (purple) and NLRP3 (green) immunostaining and the colocalization analyses of Iba-1 and NLRP3 in SNc of 12-month-old Nurr1^cKO mice and Nurr1^cWT mice. Scale bar = 20 μm. B The proportion of Iba1-positive and NLRP3-positive microglia in the SNc was quantified. Data are presented as the means ± SD. Unpaired Student T test, **p < 0.01. C Representative images with TH (red), Iba-1 (purple) and Caspase-1 (green) immunostaining and the colocalization analyses of Iba-1 and Caspase-1 in SNc of the mice. Scale bar = 20 μm. D Quantification of the proportion of Iba1-positive and Caspase-1-positive microglia in the SNc of Nurr1^cKO and Nurr1^cWT mice. Data are presented as the means ± SD. Unpaired Student T test, **p < 0.01. E Triple-label immunofluorescence of TH (red), Iba-1 (purple) and ASC (green) in SNc of the mice. Scale bar = 20 μm. F The proportion of Iba1-positive and ASC-positive microglia in the SNc was quantified. n = 3 mice per group. G The expression level of NLRP3 in PBMCs and microglia of Nurr1^cKO and Nurr1^cWT. H The level of IL-1β in the midbrain of Nurr1^cKO and Nurr1^cWT mice. I The level of IL-1β in the plasma of Nurr1^cKO and Nurr1^cWT mice. Data are presented as the means ± SD. **p < 0.01, ***p < 0.001. Unpaired Student T test