Fig. 1

LPS exposure increases GPR17 expression in the mouse brain. The mice were injected with LPS (0.25 mg/kg, i.p.) for 7 days, and the expression of GPR17 in the brain was detected by Western blot, RT-PCR, and immunofluorescent staining after injection. A Representative GPR17 protein bands in the hippocampus, cortex, hypothalamus, and VTA were detected after LPS exposure. Quantification of GPR17 protein (B) and mRNA (C) levels in the brain. D Representative images of immunofluorescent staining of GPR17 (red), NeuN (green), and DAPI (blue) in the hippocampal DG from saline mice and LPS mice. Scale bar, 100 μm. All statistics were conducted using a paired t test to account for matched control and LPS-treated mice. Values shown are expressed as mean ± SEM; n = 4 mice/group. *P < 0.05, **P < 0.01 versus LPS