Fig. 4

Knockdown and inhibition of hippocampal GPR17 inhibits the production of amyloidogenesis and oxidative response in LPS-treated mice. A Representative protein bands of APP and BACE1 in the hippocampus of lentivirus groups were shown. β-Actin was used as an internal control, and relative protein levels were quantified by densitometry analysis using Image J software. Quantification of the changes of APP (B) and BACE1 (C) protein in the hippocampus were presented as the ratio (in percentage) of the Veh + Veh group. D Levels of Aβ in the brain. E AchE activity, F ChAT activity, and levels of Ach G in the mouse brain. H Representative images of APP and BACE1 in the hippocampus of cangrelor groups were shown. Quantification of APP (I) and BACE1 (J) were presented as the ratio (in percentage) of the Veh + Veh group. K Levels of Aβ in the brain. L AchE activity, M ChAT activity, and levels of Ach N in the mouse brain. The levels of MDA (O) and SOD (P) in the hippocampus of lentivirus groups were shown. The levels of MDA (Q) and SOD (R) in the hippocampus of cangrelor groups were shown. Data shown are expressed as mean ± SEM; n = 4 mice/group. *P < 0.05, ** P < 0.01 versus Veh + LPS