Fig. 6

Knockdown and inhibition of hippocampal GPR17 ameliorated synaptic impairment and neuron apoptosis in LPS-treated mice. A Representative bands of PSD-95, SYN, cleaved caspase-3, Bcl-2, and Bax in the hippocampus of lentivirus groups were shown. Quantification of PSD-95 (B), SYN (C), cleaved caspase-3 (D), and (E) Bcl-2/Bax protein levels are expressed as the fold difference relative to the control group. F Representative bands of PSD-95, SYN, cleaved caspase-3, Bcl-2, and Bax in the hippocampus of cangrelor groups were shown. Quantification of PSD-95 (G), SYN (H), cleaved caspase-3(I), (J) Bcl-2/Bax protein levels is expressed as the fold difference relative to the control group. Protein band intensity was normalized to β-actin. Representative photographs of TUNEL-positive cells in the hippocampal DG of the LV-GPR17–shRNA (K) and cangrelor (M) groups. L, N Quantitative analysis of TUNEL-positive cells. Values shown are expressed as mean ± SEM; n = 4 mice/group. *P < 0.05, **P < 0.01 versus Veh + LPS. Scale bar, 100 μm