Fig. 7

Knockdown and inhibition of hippocampal GPR17 suppresses LPS-activated NF-κB-CREB/BDNF signaling pathway in mice. A Representative bands of NF-κB p65, p-CREB, and BDNF in the hippocampus of lentivirus groups. Quantification of NF-κB p65 (B), p-CREB (C), and BDNF (D) protein levels is expressed as the fold difference relative to the control group. (E) Representative bands of NF-κB p65, p-CREB, and BDNF in the hippocampus of cangrelor groups. Quantification of NF-κB p65 (F), p-CREB (G), and BDNF (H) protein levels in the hippocampus. The band densities were normalized to the band density of the loading control actin. The extraction process of nuclear protein in mouse hippocampus as described above. Data shown are expressed as mean ± SEM; n = 4 mice/group. *P < 0.05, ** P < 0.01 versus Veh + LPS