Fig. 1

TRIM45 expression in BV2 cells and the hippocampus is upregulated by LPS. a Experimental design. Six- to eight-week-old male C57BL/6J mice were subjected to Sham treatment or intraperitoneal injection of LPS. The hippocampus was extracted at 24 h. MWM tests were carried out from the 7th to the 11th day. b BV2 cells were treated with LPS (1 µg/mL) for 6 h and then treated with ATP (5 mM) for 30 min. The cells and culture medium were collected for testing. c Distribution and analysis of TRIM45 in the mouse whole-brain atlas. d qRT-PCR analysis of TRIM45 expression in cells induced by LPS + ATP. BV2 (mouse microglia), MA (mouse astrocytes), HT22 (mouse hippocampal neurons) cells; n = 3 per group. e The levels of TRIM45 in BV2 cells were measured by western blotting; n = 3 per group. f Immunofluorescence analysis of TRIM45 expression in BV2 cells. Scale bars, 25 µm; n = 4 per group. g Distribution and scale of TRIM45 in the hippocampal tissue of mice. h Immunofluorescence analysis of TRIM45 expression in different regions of the hippocampus; scale bars, 50 µm. i Immunofluorescence analysis of TRIM45 expression in the hippocampal CA1 region of adult mice subjected to LPS for 24 h; scale bars, 50 µm. j, k TRIM45 expression in the hippocampus was analysed by western blotting and qRT-PCR. Data in d, e, f were analysed by unpaired t test. Data are presented as the mean ± S.E.M. from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001