Fig. 2

TRIM45 promotes NLRP3 signalling activation in BV2 cells induced by LPS + ATP. a Volcano graph of the control group and transfected group. The blue dots represent low expression, while the red dots represent high expression. b Pathway analysis of the enriched DEGs in GSE76328. c Gene set enrichment analysis of GSE76328. d Western blot analysis of pyroptosis-related proteins and TRIM45 in BV2 cells. e qRT-PCR analysis of IL-1β and IL-18 mRNA levels in BV2 cells; n = 3 per group. f BV2 cell secretion of IL-1β was determined by ELISAs; n = 3 per group. g Flow cytometry was used to analyse BV2 pyroptosis; n = 3 per group. h, i Immunofluorescence analysis of NLRP3, Gsdmd, and IL-1β levels in BV2 cells. Scale bars, 25 μm; n = 3 per group. Data in e–i were analysed by one-way ANOVA followed by Dunnett’s post hoc test. Data are presented as the mean ± S.E.M. from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001