Fig. 3

TRIM45 regulates autophagy proteins associated with NLRP3. a qRT-PCR analysis of p62, Atg5, and beclin1 mRNA levels in BV2 cells; n = 3 per group. b The autophagy proteins p62, beclin1, Atg5 and LC3 in BV2 cells were measured by western blotting. c Changes in ROS in BV2 cells in the different groups; n = 3 per group. d JC-1 staining to detect mitochondrial membrane potential and cellular morphology was examined by a phase-contrast microscope. (scale bar = 75 μm); n  ≥ 3 per group. Data in a, c, d were analysed by one-way ANOVA followed by Dunnett’s post hoc test. Data are presented as the mean ± S.E.M. from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001