Fig. 5

TRIM45 regulates the NLRP3 pathway in an Atg5-dependent manner. a, b Western blot analysis and quantification of NLRP3, Gsdmd-N, Atg5 and IL-1β levels in BV2 cells transfected with siTRIM45 or HA-TRIM45 and stimulated by LPS + ATP; n = 3 per group. c BV2 cells were infected with siTRIM45 and Flag-Atg5; n = 4 per group. d BV2 cells were infected with HA-TRIM45 and siAtg5; n = 4 per group. e, f Statistical analysis of Figures c and d. g, h Flow cytometry was used to analyse BV2 pyroptosis; n = 3 per group. Data in a, b, h were analysed by one-way ANOVA followed by Dunnett’s post hoc test. Data in c, d were analysed by unpaired t test. Data are presented as the mean ± S.E.M. from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001