Fig. 6

Inhibiting TRIM45 can reduce neuronal injury in SAE. a BV2 cells were treated with siTRIM45, cocultured with neurons in a transwell system and subjected to LPS + ATP. b Levels of Caspase-1 and Cl-Caspase-1 in HT22 cells were measured by western blotting; n = 3 per group. c Immunofluorescence analysis of Cl-Caspase-1 levels in HT22 cells. Scale bars, 20 um; n = 3 per group. d Apoptosis in HT22 cells was detected by flow cytometry; n = 3 per group. e Neuronal damage in the hippocampal region was assessed by Nissl staining. Scale bar, 50 µm; n = 3 per group. Data in b, d, e were analysed by one-way ANOVA followed by Dunnett’s post hoc test. Data in c were analysed by unpaired t test. Data are presented as the mean ± S.E.M. from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001