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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Hypothermia combined with extracellular vesicles from clonally expanded immortalized mesenchymal stromal cells improves neurodevelopmental impairment in neonatal hypoxic-ischemic brain injury

Fig. 2

Intranasal ciMSC-EV therapy overcomes limited effects of HT on secondary HI-induced neurodegeneration in the striatum. C57BL/6 mice were exposed to HI on postnatal day 9 (P9) followed by 4 h HT or NT. ciMSC-EVs were delivered intranasally on day 1, 3 and 5 after HI. Western blot analyses with tissue lysates of ipsilateral hemispheres from 160-µm-thick tissue sections derived from the striatal level were performed for NeuN (A) and MAP2 (B) 7 days after HI. Data were normalized to the reference protein GAPDH and to sham animals. The number of neurons positively stained for NeuN (C) was quantified via immunohistochemistry in the striatum (D) and cortex (E). Representative images in A and B were cropped and scaled from original full length western blots provided in Additional file 1: Fig. S4. Representative images in C are derived from the striatum (scale bar 100 µm). n = 11 (sham), n = 10 (NT), n = 9 (HT), n = 11 (HT + EV), *p < 0.05, **p < 0.01, ***p < 0.001. HI = hypoxia–ischemia, NT = normothermia/vehicle, HT = hypothermia/vehicle, HT + EV = hypothermia/ciMSC-EV

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Journal of Neuroinflammation

ISSN: 1742-2094