Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Hypothermia combined with extracellular vesicles from clonally expanded immortalized mesenchymal stromal cells improves neurodevelopmental impairment in neonatal hypoxic-ischemic brain injury

Fig. 3

HT combined with ciMSC-EV treatment protects from vascular injury and promotes endothelial cell proliferation. Vessel densities, vascular injury and endothelial proliferation were determined in the cortex and striatum of postnatal day 16 (P16) C57BL/6 mice subjected to HI on P9, followed by 4 h NT or HT. Repetitive intranasal ciMSC-EV delivery was performed on day 1, 3 and 5 after HI. Vascular density and injury were determined in CD31 (green) and pan-Laminin (red)-stained tissue sections (A, scale bar: 50 µm) at day 7 after HI. Vessel densities (B) and endothelial basal lamina disruption (C) were quantified in the striatum and cortex. Total and endothelial cell proliferation was analyzed via immunohistochemistry for CD31 (green) and the proliferation marker Ki67 (red) in the striatum and cortex (D, arrows indicate CD31/Ki67 double positive cells, scale bar: 50 µm). Representative images in A and D are derived from the striatum. n = 11 (sham), n = 10 (NT), n = 9 (HT), n = 11 (HT + EV), *p < 0.05, **p < 0.01, ***p < 0.001. HI = hypoxia–ischemia, NT = normothermia/vehicle, HT = hypothermia/vehicle, HT + EV = hypothermia/ciMSC-EV

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094