Fig. 4

Depletion of microfold cells increased the count of brain infiltrating T_H2 cells in 5xFAD mice. A Gating strategy for detection of brain infiltrating CD45^highCD4⁺ (CD4⁺ T), CD45^highCD4⁺IFN-γ⁺ (T_H1) cells, and CD45^highCD4⁺IL-4⁺ (T_H2) cells. B–D Graph displaying the calculated percentage of infiltrating CD45^highCD4⁺ (CD4⁺ T) cells B, CD45^highCD4⁺IFN-γ⁺ (T_H1) cells C, and CD45^highCD4⁺IL-4⁺ (T_H2) cells D in WT, WT/Spib^−/−, 5xFAD, and 5xFAD/Spib^−/− mouse brains (n = 13–17 per group). E Histograms are representative of B220⁺ B cells at the brain. F Graph displaying the calculated percentage of B220⁺ B cells in WT, WT/Spib^−/−, 5xFAD, and 5xFAD/Spib^−/− brains (n = 4–6 per group). Bars represent as mean ± standard deviation. Statistical analysis included the two-way ANOVA and Tukey’s post hoc test. ^#P < 0.05, ^###P < 0.001, and ^####P < 0.0001 vs. WT mice. **P < 0.01 vs. 5xFAD mice