Fig. 1

Expression of RIP2 was up-regulated after cerebral ischemia. A Western blot of RIP2 expression in ischemic brain tissue from WT mice after 6 h, 12 h, 24 h, 48 h and 72 h middle cerebral artery occlusion (MCAO). n = 6 mice per group, one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05 compared with sham group. B, C Immunofluorescence of RIP2 in mouse ischemic cortex after 24 h MCAO. Double immunofluorescence of RIP2 (red) and Iba1 (microglial cell marker, green) (B), GFAP (astrocyte marker, green) (C) were performed. Scale bars: 20 μm. D Western blot of RIP2 in BV2 cells subjected to 90 min OGD and 2 h, 6 h, 12 h, 24 h reoxygenation. Results are representative of three independent experiments, one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05 compared with control group. E Western blot of RIP2 in primary cultured astrocytes cells subjected to 90 min OGD and 2 h, 6 h, 12 h, 24 h reoxygenation. Results are representative of three independent experiments, one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05 compared with control group. F, G Representative immunofluorescence image of RIP2 in BV2 cells and primary cultured astrocytes. Double immunofluorescence of RIP2 (red) and Iba1 (microglial cell marker, green) (F), GFAP (astrocyte marker, green) (G) were performed. Scale bars: 20 μm