Fig. 2

GSK2983559 improved stroke outcomes after cerebral ischemia. WT mice were subjected to 24 h MCAO. GSK2983559, an inhibitor of RIP2 was intraventricularly administered to mice 30 min before MCAO. A Neurological deficit scores. n = 8 mice per group, two-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05 compared with indicated group. B Representative photographs of coronal brain sections following infarction, stained with 2, 3, 5-triphenyltetrazolium chloride (TTC). C Summary of cerebral infarct volume in brains. The infarct volume was expressed as the percentage of the contralateral hemispheric area. n = 8 mice per group, two-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05 compared with indicated group. Western blot analysis of Phospho-NF-κB p65 and IκBα protein levels in BV2 cells (D–F) and primary cultured astrocytes (H–J) after OGD/R. ELISA analysis of proinflammatory cytokine (IL-6 and TNF-a) production in supernatant of BV2 cells (G) and primary cultured astrocytes subjected to OGD/R (K). Results are representative of three independent experiments, one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05 compared with indicated group