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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: OTUD1 ameliorates cerebral ischemic injury through inhibiting inflammation by disrupting K63-linked deubiquitination of RIP2

Fig. 4

OTUD1 interacted with RIP2. A IP analysis of the endogenous interaction of OTUD1 with RIP2 in BV2 cells after the treatment of 90 min OGD and 24 h reoxygenation (OGD/R). B Representative images of laser scanning confocal microscopy for RIP2 (red) and OTUD1 (green) in BV2 cells stimulated by OGD/R. Scale bars: 10 μm. C IP analysis of the exogenous interaction of OTUD1 with RIP2. HEK293T cells were transfected with plasmids expressing FLAG–OTUD1 and His-RIP2. D Representative images of laser scanning confocal microscopy for His (red) and Flag (green) in HEK293T cells. Scale bars: 10 μm. E Schematic diagram of RIP2 and its truncation mutants. F Myc-tagged RIP2 or its mutants and Flag-OTUD1 were individually transfected into HEK293T cells. The cell lysates were immunoprecipitated with an anti-Myc antibody and then immunoblotted with the indicated antibodies

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Journal of Neuroinflammation

ISSN: 1742-2094