Fig. 6

OTUD1 alleviated cerebral ischemic injury in mice. WT and OTUD1^−/−mice were subjected to the MCAO for 24 h. A Neurological deficit score. n = 8 mice per group, two-tailed unpaired t test, *P < 0.05 compared with MCAO group of WT mice. B Representative photographs of coronal brain sections following infarction, stained with 2, 3, 5-triphenyltetrazolium chloride (TTC). C Summary of cerebral infarct volume in brains. The infarct volume was expressed as the percentage of the contralateral hemispheric area. n = 8 mice per group, two-tailed unpaired t test, *P < 0.05 compared with MCAO group of WT mice. D Representative photomicrographs of H&E staining in the cortex and hippocampus. Scale bars: 20 μm. E Representative images of apoptosis based on TUNEL assay in brain tissue. Scale bars: 20 μm. n = 4 mice per group, two-way ANOVA with Tukey’s multiple comparisons test, *P < 0.05 compared with indicated group