Fig. 7

OTUD1 suppressed the inflammatory response by decreasing the ubiquitination of RIP2 in cerebral ischemia. A–C Western blot analysis of p-NF-κB p65 and IκBα protein levels in WT or OTUD1^−/− mice after 24 h MCAO. n = 6 mice per group, two-way ANOVA with Tukey’s multiple comparisons test, *P < 0.05 compared with MCAO group of WT mice. D–F mRNA levels of IL-1β, IL-6 and TNF-α were measured by real-time PCR in brain tissue. Results are representative of three independent experiments, two-way ANOVA with Tukey’s multiple comparisons test, *P < 0.05 compared with MCAO group of WT mice. G IP analysis of the endogenous ubiquitination of RIP2 in WT or OTUD1^−/− mice with or without the treatment of MCAO