Fig. 2
YKL-40 induces neuronal toxicity and dendritic degradation. Primary neurons were established from E17.5 wild type mice. A–D MTS assay was used to determine cell viability of DIV14 primary cultured cortex (Cor) & hippocampus (Hip) neurons treated with Aβ1-42 and/or YKL-40 for 48 or 72 h. Data are mean ± SEM. n = 4 to 12. One-way ANOVA with Tukey’s post hoc comparisons. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control. E Confocal images (Mag. 10X) of primary neurons immuno-stained with an anti-MAP2 (cyan signal) antibody. Scale bars, 100 μm. F MAP2 mean surface area was quantified. Data are mean ± SEM. One-way ANOVA with Tukey’s post hoc comparisons. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control. G–I Sholl Analysis (Image J) of dendritic intersection number from tracing images. Data are mean ± SEM of 50 to 70 neurons. Two-way ANOVA with Tukey’s post hoc comparisons. **p < 0.01, ***p < 0.001 compared to control